| 张恒斌,耿芳,马凤仓.基于明胶/海藻酸钙的止血水凝胶的制备及性能研究[J].有色金属材料与工程,2025,46(6):31-40. |
| 基于明胶/海藻酸钙的止血水凝胶的制备及性能研究 |
| Preparation and performance of hemostatic hydrogel based on gelatin/calcium alginate |
| |
| DOI：10.13258/j.cnki.nmme.20240406001 |
| 中文关键词: 止血材料 水凝胶 明胶 海藻酸钙 多巴胺 |
| 英文关键词:hemostatic materials hydrogels gelatin calcium alginate dopamine |
| 基金项目:国家自然科学基金资助项目（51771119）；上海市自然科学基金资助项目（17ZR1419600） |
|
| 摘要点击次数: 834 |
| 全文下载次数: 241 |
| 中文摘要: |
| 止血水凝胶材料因其良好的生物相容性而具有广阔的临床应用前景。但现有的止血水凝胶材料往往缺乏组织黏附性和理想的力学性能。受海洋贻贝启发，将贻贝黏蛋白中的多巴胺（dopamine，DA）接枝到明胶（gelatin，Gel）分子上，合成出具有组织黏附性的多巴胺改性明胶（Gel-DA）水凝胶。再将海藻酸钙（calcium alginate，CA）与Gel-DA构建出具有互穿聚合物网络（interpenetrating polymer networks，IPN）结构的Gel-DA/CA水凝胶。根据水凝胶中CA的含量，分别将其命名为Gel-DA/CA-0.05、Gel-DA/CA-0.10、Gel-DA/CA-0.15。测试水凝胶的组织黏附性、力学性能和止血性能等，以探究水凝胶中CA的含量对其性能的影响。试验结果表明，Gel-DA和Gel-DA/CA（0.05、0.10、0.15）水凝胶对猪皮的组织黏附强度分别为18.32、23.39 kPa，均高于明胶的(4.68±0.07) kPa。Gel-DA/CA-0.15在60%的压缩应变下表现出最强的力学强度，其所受应力为259.17 kPa，远高于Gel-DA水凝胶所受94.65 kPa的应力。体外凝血测试中，Gel-DA/CA-0.15水凝胶表现出较优的止血性能。可在346 s内凝固血液，且在相同时间下具有较低的凝血指数（blood clotting index，BCI）。 |
| 英文摘要: |
| Hemostatic hydrogel materials have broad clinical application prospects due to their excellent biocompatibility. However, existing hemostatic hydrogel materials often lack tissue adhesion and ideal mechanical properties. Inspired by marine mussels, dopamine (DA) from mussel adhesive proteins is grafted onto gelatin (Gel) molecules to synthesize dopamine-modified gelatin (Gel-DA) hydrogels with tissue adhesion. Subsequently, calcium alginate (CA) is combined with Gel-DA to construct Gel-DA/CA hydrogels with an interpenetrating polymer network (IPN) structure. Based on the content of calcium alginate in the hydrogels, they are named Gel-DA/CA-0.05, Gel-DA/CA-0.10, and Gel-DA/CA-0.15, respectively. The tissue adhesion, mechanical properties, and hemostatic performance of the hydrogels were tested to investigate the influence of calcium alginate content on their properties.
Experimental results show that the tissue adhesion strengths of Gel-DA and Gel-DA/CA (0.05, 0.10, 0.15) hydrogels on pig skin are 18.32 kPa and 23.39 kPa, respectively, both higher than that of Gel (4.68±0.07) kPa. Gel-DA/CA-0.15 exhibits the strongest mechanical strength at a compression strain of 60%, with a stress of 259.17 kPa, which is much higher than the 94.65 kPa of Gel-DA hydrogels. In in vitro coagulation tests, Gel-DA/CA-0.15 shows optimal hemostatic performance: it can clot blood within 346 s and has a lower blood clotting index (BCI) at the same time. |
| HTML 查看全文 查看/发表评论 下载PDF阅读器 |
|
|
|
