| 彭申,周柳艳,陈爱英,毛燕飞,任荣荣.基于万古霉素高效还原Ag纳米粒子体系的分光光度法检测[J].有色金属材料与工程,2024,45(4):41-50. |
| 基于万古霉素高效还原Ag纳米粒子体系的分光光度法检测 |
| Spectrophotometric determination of vancomycin based on its highly-efficient reduction performance to Ag nanoparticle |
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| DOI：10.13258/j.cnki.nmme.20231203001 |
| 中文关键词: 万古霉素 银纳米粒子 光谱法 检测 |
| 英文关键词:vancomycin Ag nanoparticles spectrophotometric method detection. |
| 基金项目:国家自然科学基金面上资助项目（51771121） |
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| 中文摘要: |
| 万古霉素 （vancomycin，Van ）是临床实践中常用的抗生素，其用于预防传染病，但在环境水微量污染物检测中常常被忽略。利用溶剂热反应过程中 Ag+ 选择性氧化 Van 中酚类羟基团特性，开发了一种利于分光光度法检测废水中 Van 的方法。探讨了反应温度和时间等对氧化还原反应的影响，建立了 Van 质量浓度（ concentration，C ）与 Ag 纳米粒子溶液吸光度（ absorbance，A ）之间的线性关系。发现 C 与 A 呈双线性关系：在线性浓度范围为 1～15 μg/mL 时，线性关系为 $ {A}_{1}=0.001\;7+0.012{C}_{1} $， 检测限为 0.5 μg/mL ；在 20～40 μg/mL 时， $ {A}_{2}=0.11+0.005\;5{C}_{2} $ 。该方法成功应用于废水中 Van 检测，回收率为 96.0% ～104.5% ，废水中可能存在其他物质或颗粒，干扰了 Van 的测量，导致回收率计算结果超过 100%。 |
| 英文摘要: |
| Vancomycin (Van) is a typical antibiotic in clinical practice to prevent infectious diseases, which is generally neglected in the detection of trace pollutant in environmental water. A spectrophotometric method is developed to detect Van in waste water based on Ag nanoparticles, which involves a selective reduction of Ag+ by the phenolic-hydroxyl groups of Van to obtain different concentrations of Ag nanoparticles during the solvothermal reaction. By optimizing experimental conditions of reaction temperature and time, a bi-linear relationship between Van concentration (C) and absorbance (A) of Ag solution is established, represented by A1=0.0017+0.012C1 in a linear concentration of 1-15 μg/mL with a detection limit of 0.5 μg/mL, and A2=0.11+0.0055C2 of 20-40 μg/mL. This method successfully applied to detect Van in waste water with a recovery rate of 96.0%-104.5% possibly due to other substances or particles in the wastewater that interfere with the measurement of Van and cause the calculated recovery rate exceeding 100%. |
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