| 田悦诚,何斌,王丁.Au纳米颗粒形貌调控工艺及SERS性能研究[J].有色金属材料与工程,2025,46(4):28-40. |
| Au纳米颗粒形貌调控工艺及SERS性能研究 |
| Tailoring Au nanoparticle morphology for enhanced SERS performance: process optimization and characterization |
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| DOI：10.13258/j.cnki.nmme.20240107001 |
| 中文关键词: 急性心肌梗死 miRNA 表面增强拉曼散射 金纳米颗粒 生物检测 |
| 英文关键词:acute myocardial infarction miRNA surface-enhanced Raman scattering gold nanoparticles bioassays. |
| 基金项目:上海市自然科学基金面上项目(19ZR1435200) |
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| 中文摘要: |
| 快速、准确地检测急性心肌梗死（acute myocardial infarction，AMI）可以提高患者的生存机会。除了肌钙蛋白（cardiac troponin I，cTnI）和肌红蛋白（myoglobin，MyO）等生物标志物，miRNA（microRNA）已被证明是心肌梗死早期诊断的重要生物标志物。然而，目前常规的免疫检测方法如实时荧光定量聚合酶链式反应（polymerase chain reaction, PCR）的检测灵敏度有限且耗时，不足以完成miRNA高灵敏的定量分析。表面增强拉曼散射（surface enhanced Raman scattering，SERS）是一种出色的指纹诊断技术，具有超灵敏度、快速响应和定性定量分析的能力，对其有效设计和研究有望实现AMI的高效诊断。通过调控贵金属Au的不同形貌，研究了调控因素与性能之间的关联，并基于电磁场增强机制和化学增强机制，设计了简便易制备的高效金 纳米球（gold nanospheres，Au NSs） SERS生物检测基底，且成功应用于miRNA的梯度浓度测试，结果显示出了超低的检测限与较高的灵敏度。 |
| 英文摘要: |
| Rapid and accurate detection of acute myocardial infarction (AMI) can significantly enhance patient survival rates. While cardiac troponin I (cTnI) and myoglobin (MyO) serve as conventional biomarkers, miRNA（MicroRNA） has emerged as a crucial early diagnostic biomarker for heart attacks. However, current routine immunodetection methods, such as real-time fluorescence quantitative polymerase chain reaction (PCR), exhibit limited sensitivity and are time-consuming, hindering the achievement of high-sensitivity quantitative miRNA analysis. Surface Enhanced Raman Scattering (SERS) stands out as an exceptional fingerprint diagnostic technology, offering ultra-sensitivity, rapid response, and qualitative and quantitative analytical capabilities. In this study, the correlation between performance and modulation factors was explored through the manipulation of various morphologies of the noble metal Au. Leveraging the electromagnetic field enhancement and chemical enhancement mechanisms, we designed a simple and easily manufacturable efficient gold nanospheres (Au NSs）NSs SERS biosensing substrate. This substrate was successfully employed for the gradient concentration testing of miRNA, demonstrating remarkably low detection limits and high sensitivity. |
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